刊期:双月刊
主管单位:中国科学院
主办单位:中国科学院动物研究所,中国昆虫学会
地址:北京市朝阳区北辰西路1号院5号中国科学院动物研究所
邮编:100101
电话:010-64807137
传真:010-64807137
E-Mail:entom@ioz.ac.cn
刊号:ISSN 2095-1353
        CN 11-6020/Q
国内发行代号:2-151
国际发行代号:BM-407
发行范围:国内外公开发布
定价:138元/册
定价:828元/年
银行汇款:中国工商银行北京海淀西区支行
户名:中国科学院动物研究所
帐号:0200 0045 0908 8125 063

您所在位置:首页->过刊浏览->2023年60卷第5期



基于微卫星标记的斜纹夜蛾 多次交配的亲权鉴定
Using microsatellite markers to determine the paternity of Spodoptera litura (Fabricius)
狄雪塬,李昊熙,严 斌,杨茂发
点击:267次 下载:8次
DOI:10.7679/j.issn.2095-1353.2023.131
作者单位:贵州大学昆虫研究所
中文关键词:斜纹夜蛾;微卫星标记;亲权鉴定;多次交配;精子竞争
英文关键词: Spodoptera litura; microsatellite marker; paternity identification; multiple mating; sperm competition
中文摘要:

【目的】 明确斜纹夜蛾Spodoptera litura交配次数及交配顺序对父权分配的影响。【方法】 利用微卫星标记对不同多次交配处理后的斜纹夜蛾子代进行亲权鉴定。实验筛选出5对微卫星标记,共采集271头斜纹夜蛾样本,其中母本21头,父本40头,子代210头。【结果】 微卫星标记的等位基因数、期望杂合度、多态信息含量的平均值分别为4.800、0.647、0.596。鉴定置信度均高于80%,其中106头的鉴定置信度超过95%。斜纹夜蛾雌虫与2头雄虫分别交配1次,后交配雄虫的子代父权比例为78%;雌虫与1头雄虫交配2次,再与另一头雄虫交配1次,2头雄虫的子代父权比例均为50%;雌虫与1头雄虫交配1次,再与另一头雄虫交配2次,后交配雄虫的子代父权比例高达90%;雌虫与3头不同雄虫分别交配1次,3头雄虫子代父权比例分别为5%、20%和75%。【结论】 斜纹夜蛾交配次数和交配顺序均会影响父权比例。交配次数可以增加子代的父权比例,后交配的雄性拥有更高的父权比例。

英文摘要:

[Objectives]  To investigate the effect of mating frequency and male mating order on the paternity of Spodoptera litura, an important global agriculture and forestry pest. [Methods]  Paternity was determined using five pairs of microsatellite markers. 271 samples were collected, including 21 from female parents, 40 from male parents and 210 from offspring. [Results]  The average number of alleles, expected heterozygosity and polymorphism content information were 4.800, 0.647 and 0.596, respectively. Confidence in estimated paternity was overall > 80%; > 95% for 106 offspring. If a female mated once with two males, 78% of the resultant offspring were the progeny of the last male to mate. If a female mated with a male twice with a male, then with another male once, 50% of the offspring were the progeny of each male. If a female mated with a male once, then with another male twice, 90% of the offspring were the progeny of the last male to mate. If a female mated once with three different males, 5%, 20% and 75% of the offspring were the progeny of the first, second and last males to mate, respectively. [Conclusion]  Both the frequency and order of mating affect paternity in S. litura. Males that mate more than once, and those that mate last, have more progeny than those that only mate once or that aren’t the last to mate.

读者评论

      读者ID: 密码:   
我要评论:
版权所有©2024应用昆虫学报》编辑部 京ICP备10006425号
本系统由北京菲斯特诺科技有限公司设计开发
您是本站第9197267名访问者