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茶皂素对飞蝗解毒酶与保护酶活性的影响
Effects of tea saponin on the detoxification and protective enzyme activity of Locusta migratoria
王铭铭,张勇娟,崔国盈,季 荣,张永军,何 岚
点击:194次 下载:10次
DOI:10.7679/j.issn.2095-1353.2023.134
作者单位:新疆师范大学生命科学学院
中文关键词:茶皂素;解毒酶;保护酶;飞蝗
英文关键词:tea saponin; detoxifying enzyme; protective enzyme; Locusta migratoria
中文摘要:

【目的】 明确茶皂素对飞蝗Locusta migratoria解毒酶与保护酶活性的影响,以阐明茶皂素对飞蝗毒性的作用机制。【方法 通过腹部注射低、中和高3种不同浓度的茶皂素溶液,比较不同浓度茶皂素对飞蝗谷胱甘肽-S-转移酶(GST)、羧酸酯酶(CarE)、细胞色素P450CYP450)、过氧化物酶(POD)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性的影响,分析低浓度茶皂素溶液处理0122448 h后试虫体内解毒酶与保护酶活性的变化规律。【结果】 3种解毒酶活性在低浓度溶液处理后即迅速增加,但GST活性随后被抑制,CarE活性在中浓度溶液处理时最高,CYP450活性随茶皂素浓度的增加而持续增加。随茶皂素处理时间延长,CarECYP450活性在处理12 h后显著增加,GST活性在处理24 h后显著增加。PODCAT在低浓度溶液处理下活性最高,SOD活性在中浓度溶液处理时开始显著增加。3种保护酶在低浓度溶液处理24 h时开始显著增加,其中,PODSOD活性在茶皂素处理48 h后持续增高,而CAT活性在处理48 h后下降。【结论】茶皂素可影响飞蝗解毒酶和保护酶活性,从而对飞蝗产生毒性作用。

英文摘要:

 [Objectives]  To investigate the effects of tea saponin on detoxification and protective enzyme activity of Locusta migratoria. [Methods]  The effects of different concentrations of tea saponin on the activity of glutathione-S-transferase (GST), carboxylatetasse (CarE), cytochrome P450 (CYP450), peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) were compared by injecting low, medium and high concentrations of tea saponin solution into the abdomen of adult L. migratoria and monitoring changes in detoxification and protective enzyme activity after 0, 12, 24 and 48 h. [Results]  The activity of the three detoxification enzymes increased rapidly in the low concentration treatment group, but GST activity was subsequently inhibited, and CarE activity was highest in the medium concentration treatment group. The activity of CYP450 increased with tea saponin dosage. CarE and CYP450 activity increased significantly after 12 h, and the activity of GST increased significantly after 24 h. POD and CAT had the highest activity in the low-concentration treatment group, whereas SOD activity increased significantly in the medium concentration treatment group. Protective enzyme activity began to increase significantly after 24 h in the low concentration treatment group. POD and SOD activity continued to increase, but CAT activity decreased, after 48 h. [Conclusion]  Tea saponin can affect the activity of both detoxifying and protective enzyme in Locusta migratoria.

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