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章鱼胺和酪胺及其受体拮抗剂对 普通大蓟马子代性比的影响
The role of octopamine, tyramine, and three antagonists on the offspring sex ratio of Megalurothrips usitatus (Bagrall) (Thysanoptera: Thripidae)
陈钰晴,任英凯,叶子龙,刘袁媛,邹游兴,但建国
点击:112次 下载:7次
DOI:10.7679/j.issn.2095-1353.2023.145
作者单位:海南大学植物保护学院
中文关键词:普通大蓟马;性比;豇豆;生物胺;拮抗剂
英文关键词:Megalurothrips usitatus; sex ratio; cowpea; biogenic amines; antagonists
中文摘要:

【目的】 明确章鱼胺和酪胺对普通大蓟马Megalurothrips usitatus子代性比的影响。【方法】 在(26±1℃、相对湿度60%±5%、光周期L∶D = 14∶10条件下,采用营养液饲喂法,以10%蔗糖液(对照)和含有20 mmol∙L1章鱼胺、20 mmol∙L1酪胺、1 mmol∙L1酚妥拉明、1 mmol∙L1米安色林和1 mmol∙L1育亨宾的10%蔗糖液分别饲喂已交配1日龄雌虫48 h后,再用嫩豇豆豆荚单头饲养10 d。观察雌虫每天所产子代的若虫数量、成虫的性别与数量,计算子代的雄性比、日性比和存活率。【结果】 仅酚妥拉明处理的雄性比(0.47)显著高于对照(0.34),其日性比的大幅提高出现于产卵第3-5天和第9天。章鱼胺和酚妥拉明处理显著降低雌性子代成虫和子代成虫的数量,而育亨宾处理仅导致雌性子代成虫数显著减少。各处理对雄性子代成虫数和子代存活率都没有显著影响。【结论】 酚妥拉明能通过抑制雌性子代的产生,来提高普通大蓟马的子代雄性比。

英文摘要:

[Objectives]  To determine the effects of octopamine and tyramine on the offspring sex ratio of the bean flower thrip, Megalurothrips usitatus (Bagnall), an economically important pest of legumes. [Methods]  Under conditions of (26 ± 1) , 60% ± 5% RH and a photoperiod of 14︰10D, 1-day-old mated, adult females were fed for 48 h on either a 10% sucrose solution (control), or a 10% sucrose solution plus either 20 mmol∙L1 octopamine, 20 mmol∙L1 tyramine, 1 mmol∙L1 phentolamine, 1 mmol∙L1 mianserin, or 1 mmol∙L1 yohimbine, which was confined between two layers of stretched parafilm, then reared individually for 10 d on a section of young cowpea (Vigna unguiculata) bean pod in a glass tube. Bean pod sections were replaced daily. Replaced bean pod sections were placed into a new glass tube until offspring emerged, after which the numbers of nymphs, and their sex were recorded. Sex ratio (defined as proportion of malesand offspring survival (%) were calculated. [Results]  Only the phentolamine treatment group had a significantly higher adult offspring sex ratio (0.47) than that of the control group (0.34). Offspring sex ratios in this treatment group were significantly higher than those of the control on the 4th, 5th day and 9th day of oviposition. Both the octopamine and phentolamine treatment groups had significantly fewer adult female offspring, as well as fewer adult offspring overall. The production of female offspring was inhibited by yohimbine. None of the treatments affected the number of male offspring or the offspring survival rate. [Conclusion]  Phentolamine inhibits the production of female offspring by mated female bean flower thrips, and thereby increases sex ratio of their offspring.

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