【目的】 旨在探索兴义维蚋Simulium xingyiense实验室饲养方法，使其能在实验室内连续饲养2代。【方法】 设计制作羽化杯、交配管、喂血装置和产卵管，并改造市售小型鱼缸供蚋卵孵化及后续饲养。第1代饲养：将野外采集得到的兴义维蚋蛹置于羽化杯中饲养获得成虫；在交配管中诱导成虫交配（30 min）；将雌蚋转移至喂血装置中喂养（90 min）；将饱腹雌蚋置于产卵管中产卵；将产出的卵置于改造过的小型鱼缸中孵化，并饲养至幼虫化蛹。第2代饲养：除蛹来源于第1代饲养外，所有步骤同第1代饲养。全部实验在温度20 ℃、相对湿度70%下进行，但在喂血环节分别测试喂血培养基不同温度和成分对饱腹率和产卵率的影响。【结果】 添加ATP的血清培养基更能吸引雌蚋吸血；在喂血培养基温度为34 ℃时雌蚋饱腹率最高；兴义维蚋雌虫喜食牛血清培养基；喂血培养基内血清含量越高，饱腹雌蚋产卵率越高；在最适喂养条件下雌蚋饱腹率为25.3%，产卵率为29.4%，平均每只雌蚋产163.5个卵，蚋卵孵化率为85.5%。幼虫生长周期为13-15 d，结蛹率为10.7%；蛹经4-6 d可羽化为成虫，羽化率为95.2%。【结论】 兴义维蚋可在实验室内连续饲养2代，能够满足后续分子生物学实验要求，但幼虫结蛹率较低，提示改善幼虫饲养、提高结蛹率可能是最终建立兴义维蚋实验室品系的关键所在。

[Objectives]  To improve current rearing methods for Simulium xingyiense to enable the maintenance of two consecutive generations under laboratory conditions. [Methods]  We designed and built emergence cups, mating tubes, blood-feeding apparatus and oviposition tubes, and modified a commercially available miniature aquarium for hatching eggs and rearing successive generations. The first generation was reared as follows: pupae were collected in the field and placed in emergence cups until adults emerged. Adults were then transferred to mating tubes for 30 minutes after which females were transferred to the blood-feeding apparatus for 90 minutes. Engorged females were placed in oviposition tubes until they laid eggs, which were then hatched in the modified aquarium and reared until the larvae pupated. The second generation was reared by repeating these steps with pupae from the first generation. All experiments were carried out at a temperature of 20 ℃ and a relative humidity of 70%. The effects of different feeding media temperatures and compositions on engorgement and oviposition rates were measured and compared. [Results]  Serum medium enriched with ATP was found to be the most appealing to female flies. The highest engorgement rate was measured at a medium temperature of 34 ℃. Female flies preferred bovine serum medium, and the higher its serum content, the higher their oviposition rate. Under the above optimal conditions, the female engorgement rate was 25.3%, the oviposition rate was 29.4%, each female produced an average of 163.5 eggs and the hatching rate was 85.5%. The larval stage lasted 13-15 days with a pupation rate of 10.7%. Adults emerged after 4-6 days at an emergence rate of 95.2%. [Conclusion]  Two consecutive generations of S. xingyiense can be reared from pupae under laboratory conditions for subsequent molecular experiments. However, the pupation rate was low, so finding a way to increase this would further improve the captive rearing of this species.