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不同地理种群的亚洲小车蝗mtDNA ND1基因序列及其相互关系
Sequence analysis of mtDNA ND1 gene and relationships of geographical populations of Oedaleus asiaticu
高书晶1**,刘爱萍1,韩静玲2,闫志坚1,徐林波1,李东伟3
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DOI:
作者单位:1中国农业科学院草原研究所呼和浩特010010;2 唐山市农业科学研究院唐山063001;
中文关键词: 亚洲小车蝗, 地理种群, mtDNA, ND1基因, 遗传关系
英文关键词:Oedaleus asiaticus, geographical populations, mtDNA, ND1, genetic relationship
中文摘要:

      测定了7个不同地理种群的亚洲小车蝗Oedaleus asiaticus(Bienko)、1个近缘种及2个外群种共31个样本的mtDNA ND1基因序列,比较其同源性,计算核苷酸组成,并以槌角蝗科的宽须蚁蝗Myrmeleotettix palpalis和斑腿蝗科的鼓翅皱膝蝗 Angaracris barabensis作外群构建UPGMA和NJ分子系统树。在获得的亚洲小车蝗347 bp的序列中, A+T约占76.6%,其中22个核苷酸位点存在变异(约占所测核苷酸的7.26%)。就每个氨基酸密码子来看,第3位点的A+T含量最高。28个个体共检测出18个单倍型,单倍型多样性指数(H)为0.218,核苷酸多样性指数(Pi)为 0.0018。分化系数为0.0128~0.1573,基因流Nm均大于1;AMOVA分析显示种群间的差异有72.36%存在于群体内部,27.64%存在于群体之间。分子系统树显示, 亚洲小车蝗mtDNA ND1序列不同个体之间有一定的分歧,形成不同的簇类关系,其分枝与地理分布没有直接的对应关系,但总体上看,这种簇类关系基本上呈平行分布,没有明显的地域性差别。
 

英文摘要:

       Mitochondrial DNA ND1 gene sequences were sequenced in 31 individuals of Oedaleus asiaticu (Bienko)from 7 geographical populations in Inner Mongolia, including 1 related species and 2 outgroup species (Myrmeleotettix palpalis and Angaracris barabensis). We compared homologous sequences, calculated nucleotide composition and constructed a molecular phylogenetic tree with the Neighbor-Joining and UPGMA method. In the sequences obtained, A+T was about 76.6% and 22 nucleotide sites (about 7.26%) were substituted. A+T was much higher at the third site than the other two sites in every amino acid code. 18 hapotypes were detected in 28 individuals; hapotype diversity index(H)and mean nucleotide diversity index(Pi)reached 0.218 and 0.0018 respectively. Genetic divergence Fst was 0.0128—0.1573 and gene flow Nm was greater than 1. An AMOVA test indicated that there was 72.36% genetic variation within, and 27.64% between, populations. The phylogenetic trees suggested that different individuals from the 7 areas in Inner Mongolia were located in different clusters, but no clear relationships between haplotypes and geographical regions could be inferred. Because the branches of each cluster are parallel there is no clear difference between the different areas.

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