中华稻蝗血细胞染色方法的比较
Comparison among three staining methods to hemocytes of Oxya chinensis
王强,崔智芳,王云丽,申旭燕,张小民**
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作者单位:山西大学生命科学学院太原 030006
中文关键词:中华稻蝗, 血细胞, 染色方法
英文关键词:Oxya chinensis, hemocytes, staining method
中文摘要: 通过Giemsa、Wright和GiemsaWright混合染色3种方法,对中华稻蝗Oxya chinensis(Thunberg)的血细胞进行了染色和血细胞的形态学观察,其结果是Giemsa染液染色时间较长,对细胞核染色效果较好,细胞质界限清晰,但对细胞质中的颗粒染色较差;Wright染液染色时间较短,对细胞核和细胞质中的颗粒染色效果较好,但细胞质的界限不淸;GiemsaWright混合染色具备了2种染液的优点,时间介于两者之间,细胞核和细胞质中的颗粒染色效果较好,细胞界限清晰,该法可用于蝗虫血细胞,以及昆虫血细胞的广泛研究。
英文摘要: The staining efficiency of the Giemsa, Wright and WrightGiemsa staining methods were compared with respect to hemocytes of Oxya chinensis(Thunberg). The Giemsa method effectively stained the nucleus; cytoplasm boundaries were clear, but it was relatively time consuming and did not stain cytoplasmic granules very well. The Wright method effectively stained both nucleus and cytoplasmic granules in a relatively short time, but cytoplasm boundaries were not clear. The WrightGiemsa method had the advantages of both the Giemsa and Wright methods, including shorter staining time, good staining of nucleus and cytoplasmic granules and clear cytoplasm boundaries. This method should be used more widely in studying insect hemocytes.