Analysis of chemical components from eggs surface and female abdominal scales of Plutella xylostella
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Key Words：Plutella xylotella，GC-MS，egg surface，abdominal scales， analysis
Abstract： Volatile chemicals emanating from the eggs and adult abdominal scales of the Diamondback moth (DBM),Plutella xylostella (L.),influence the host-seeking behavior of Trichogrammatoidea bactrae Nagaraja,Trichogramma confusum Viggiani, Trichogramma pretiosum Riley and Trichogramma ostriniae Pang et Chen.In order to identify the main component of the volatile chemicals emitted by DBM eggs and adults,the hydrocarbon profile of hexane extracts collected from the surface of DBM eggs and female abdominal scales were analyzed using Gas Chromatography/Mass Spectrometry (GC-MS).Forty-eight chemical components were isolated,16 components from the eggs’ surface and 32 from female abdominal scales.The contents of these chemicals was determined by the area normalizing method after searching the NIST database and comparison with standard mass spectra.Eleven chemical components from the eggs’ surface were identified with a total mass fraction of 93.95%.Seventeen chemical components from female abdominal scales were identified with total mass fraction of 88.47%.The principal components of hexane extracts from the surface of DBM eggs and female abdominal scales were straight and branched-chain saturated hydrocarbons ranging from C-12 to C-36 with some aliphatic acid ramification.