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2012年49 No.4
Effects of Vip3Aa and Cry1Ac on enzyme activity in cottonbollworm Helicoverpa armigera larvae
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Key Words：Helicoverpa armigera，Vip3Aa，Cry1Ac，protease，detoxification enzymes
Abstract：In order to clarify the activity of Vip3Aa and provide a theoretical basis for its application in the“New toxin
strategy”，the effects of Vip3Aa and Cry1Ac on protease，detoxification enzymes were and aminopeptidase N ( APN)
activity in larvae of the cotton bollworm Helicoverpa armigera ( Hübner) were compared and the impacts of Cry1Ac +
Vip3Aa on these enzymes were investigated． The lethality of Vip3Aa was lower than that of Cry1Ac，but Vip3Aa had an
obvious inhibitory effect on larval development． Total protease and trypsin-like enzyme activity quickly increased in H．
armigera fed an artificial diet containing either Cry1Ac or Vip3Aa or Cry1Ac + Vip3Aa． However，compared to the
control，there was no significant difference in the activity of these two enzymes after 12 h of feeding on the Cry1Ac diet．
While the period during which the activity of these two enzymes increased was clearly prolonged in larvae fed on diets
containing Vip3Aa，that of the chymotrypsin-like enzyme was also higher than in the control． This indicates that the
degradation rate of Cry1Ac was faster than that of Vip3Aa，and that the enzyme systems involved in degradation could be
different． Meanwhile，the diet containing Cry1Ac and Vip3Aa together extended the degradation time． The activities of
glutathione-S-transferase and α-naphthalene acetate esterase increased in H． armigera fed on a diet containing either
Cry1Ac or Vip3Aa or Cry1Ac + Vip3Aa． This indicates that these enzymes may be involved in the detoxification of Vip3Aa
and Cry1Ac． However，Vip3Aa and Cry1Ac had little effect on aminopeptidase N activities，suggesting that the toxicity of
the latter has no relationship to APN enzyme activity．