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2012年49 No.5
Research on optimal level of calcium and phosphorus inpollen substitutes
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Key Words： Apis mellifera ligustica，pollen substitute，optimal level of calcium and phosphorus
Abstract：In order to determine the optimal levels of calcium and phosphorus required in pollen substitutes，different
levels of CaCO3 and NaH2 PO4 ·H2O were added to the pollen substitutes provided to experimental colonies of Apis
mellifera ligustica Spinola． 70 A． mellifera ligustica colonies of equal size，queen quality and age were randomly allocated
to 14 treatment groups (5 replications of each group) ． The 14th group was fed pure rape pollen ( control treatment)，the
13th group was fed diets with no CaCO3 and NaH2 PO4·H2O ( negative control treatment)，and the others were fed pollen
substitutes with different levels of CaCO3 and NaH2 PO4·H2O ( groups 1 － 12) ． The trial ran from spring to the end of the
honey locust flow． The total intake of each colony，the overall honeybee population and the emergence weight and mineral
content of adult honeybees，were monitored during the trial period． Regression analysis was used to determine the optimal
levels of CaCO3 and NaH2 PO4·H2O． The results show that maximal values of total intake for colonies，total population
size and emergence weight can be obtained at a dosage of 0 calcium and 0. 65% phosphorus． In conclusion，there is the
positive correlation between the calcium content of adult honeybees and the level of calcium and phosphorus in pollen
substitutes，but no such relationship exists for phosphorus．