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2012年49 No.6
Metabolic mechanisms of resistance to emamectincypermethrin in field populations of Spodoptera exigua
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Key Words：Spodoptera exigua，emamectin，cypermethrin，monooxygenases，glutathione S-transferases，esterases
Abstract： Three populations of Spodoptera exigua ( YN-JN，SH-FX，and JS-LH) showed high levels of resistance to
emamectin (45 to 437-fold ) and cypermethrin ( 211 to 555-fold ) compared to a susceptible SWH strain． Increasedactivity of detoxification enzymes such as monooxygenases (2. 7 to 8. 4-fold)，glutathione S-transferases (1. 9 to 8. 6-fold)and esterases (1. 6 to 5. 7-fold) were detected in these populationscompared with the SWH strain． The synergistic ratios( SRs) of the oxidase inhibitor ( PBO) and the esterase inhibitor ( DEF) to emamectin were 1. 2-4. 3 and 1. 3-7. 7respectively and the SRs of PBO and DEF to cypermethrin were 1. 8-58 and 3. 6-245 respectively． The glutathione Stransferaseinhibitor (DEM) displayed no significant synergism with these two insecticides． These results indicate thatenhanced detoxification by bothesterases and oxidases are important resistance mechanisms to emamectin andcypermethrin in S． exigua，and that enhanced detoxification is associated with increased esterase and oxidase activitybutnotincreasedglutathione S-transferase activity． Multiple-pesticide resistance in many insect populations makes synergisticbioassays a more reliable method of determining the contribution of metabolic detoxification to resistance to specific
insecticides．