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2012年49 No.6
cDNA cloning and mRNA expression of β-N-acetylglucosaminidase indifferent tissues of Agrotis ipsilon Hufngel
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Key Words： Agrotis ipsilon，β-N-acetylglucosaminidase，cloning，mRNA expression
Abstract：β-N-acetylglucosaminidase is an enzyme that can degrade chitin in insects． Total RNA was isolated from theprepupal stage of Agrotis ipsilon Hufngel． The cDNA sequence was cloned by RT-PCR and rapid amplification of cDNAends(RACE) ． The cDNA，2 701 base pairs in length，contained an open reading frame of 1 788 base pairs coding for a polypeptide of 595 amino acid residues with a predicted molecular weight of 68. 3 ku and pI 5. 48． The predicted proteinshared extensive similarities with those from other insects，expecially Lepidopteran insects． The cDNA sequence has beendeposited in GenBank with the accession No． GU985280． RT-PCR results indicate that β-N-acetylglucosaminidase mRNA
was expressed in the malpighian tubules，midgut，fat bodies and integument．