Rapid identification of two species of Tetradacus by PCR-RFLP
Author of the article:LIN Li-Li1;WU Jia-Jiao2;ZENG Ling1;LIANG Guang-Wen1;HU Xue-Nan1;MO Ren-Hao2
Author's Workplace:1. Laboratory of Insect Ecology, South China Agricultural University, Guangzhou 510642, China; 2. Guangdong Inspection and Quarantine Technology Center, Guangzhou 510623, China
Key Words:Bactrocera (Tetradacus) tsuneonis; Bactrocera (Tetradacus) minax;PCR-RFLP; COⅡ; ITS1
Abstract:The rapid molecular diagnostic technique for identification of Bactrocera (Tetradacus) tsuneonis and Bactrocera (Tetradacus) minax was based on PCR-RFLP of the mitochondrial DNA (mtDNA) and ribosomal DNA (rDNA). With four pairs of primers an