An effective method to extract total RNA from small insects
Author of the article:DENG Shun;ZHANG You-Jun;CHU Dong
Author's Workplace：Institute of Vegetable and Flower， Chinese Academy of Agricultural Sciences,Beijing 100081,China
Key Words：Small insects; RT-PCR; RNA extraction
Abstract：Integrated and purified RNA was extracted from aphids and other small insects, using ordinary chemicals, sodium dodecylsulfate (SDS), potassium acetate(KAc), etc. Experiments proved the extracted RNA had good quality that could be used to RT-PCR and const