Influence of 30K protein in Bombyx mori on survival BmN cells and larvae
Author of the article:TANG Ping1，2**, LI Bing3, SHEN Wei-De3, CHEN Jing-Ping1, WANG Wen-Bing1***
Author's Workplace：1.Institute of Life Sciences, Jiangsu University,Zhenjiang 212013, China; 2.School of Biology ＆ Environment Engineering，Jiangsu University of Science and Technology,Zhenjiang 212018, China; 3.School of Life Sciences, Soochow University, Suzhou 215123, China
Key Words：Bombyx mori；30K protein； baculovirus； expression； longevity
Abstract：
The 30K protein was successfully expressed in the Bombyx mori L. (Bm) cells by baculovirus system. B. mori cells and larvae were infected with the recombinant baculovirus(Bm/r-30K), respectively. The viability of cells and larvae were assayed at various times post-infection and BmPAK6 was used as a control. The viability of BmN cells infected with baculovirus(Bm/r-30K) was higher than that of the cells infected with BmPAK6 virus, the viability and surviving time of B. mori larvae were increased as well. The results indicate that over-expression of the B. mori 30K gene contributes to increase the surviving time of BmN cells and larvae.