Genetic diversity of different geographical populations of Sesamia inferens Walker by AFLP
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Author's Workplace：Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences
Key Words：Sesamia inferens Walker; geographic population; AFLP; genetic diversity
Abstract：To study the mechanism of the genetic diversity of different Sesamia inferens Walker populations, ten characteristic geographical populations from ten provinces of China were selected. With 37 pairs of AFLP selective primers, 1055 AFLP loci were recorded, of which 78.75% were polymorphic. Coefficient of gene differentiation (Gst) between populations was 0.7132, Nei’s gene diversity index (h) from 0.2969 to 0.4547 and the average was 0.3752, Shannon’s index from 0.2936 to 0.5043 and the average was 0.3888. There was high genetic identity between the 10 populations with the value greater than 0.63. NJ cluster analysis showed the ten populations are grouped into four major clades based on polymorphism of AFLP markers, of which DY, LH and ZJ populations belong to three different clades, and the other seven populations belong to one clade. The populations within each of the clades are consistently subject to similar geographic climate and near distance. However, the isolated population with longer genetic distance to all other tested populations was probably clustered into a random clade. So the sampled populations for genetic polymorphism clustering should be as many as possible and dispersed.