Optimization an artificial diet for the rice leaf-folder, Cnaphalocrocis medinalis
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Key Words： rice leaffolder, Cnaphalocrocis medinalis, artificial diet, design of quadratic orthogonal rotational combination,
Abstract： An artificial diet for the rice leaffolder, Cnaphalocrocis medinalis Guenée was optimized using an experimental design of quadratic orthogonal rotational combinations. An optimal diet recipe was obtained from a quadratic regression model taking the pupation rate of the rice leaf-folder as the dependent variable. The results show that proportions of rice leaf powder, wheat germ powder, corn powder, casein and yeast in the artificial diet affected the rate of pupation rate of rice leaf-folder larvae. The optimal proportions of these components were: rice leaf powder 3g, wheat germ powder 8 g, corn powder 4g, casein 4g and yeast 4g in 135 g of diet. When rice leaf-folder larvae were reared on this optimum diet at 27℃, 80%RH and a 16L:8D photoperiod, 25.6% of the tested neonate larvae could complete larval development and pupate in about 26.9 days. Pupal weight was about 16-22 mg. Rates of pupation and pupal weight were obviously higher, and the duration of the larval period slightly shorter, than previously reported in other studies