[Objectives]  The pink borer, Sesamia inferens (Walker) has in recent years become a major pest of rice crops that is responsible for steadily increasing crop losses. The heat shock protein 90 (HSP90) family is of great significance for the correct folding and transmission of proteins in organisms. HSP90 affects the growth and development of organisms and their responses to external stimuli. Our study’s aim was to clone the full length cDNA and genome sequences of the HSP83 gene from S. inferens, and analyze its basic characteristics. [Methods]  RT-PCR and RACE were used to clone the full-length cDNA of HSC70 gene from S. inferens. The genomic sequence was determined by genomic validation, introns were analyzed by sequence analysis and a phylogeny constructed. [Results]  The complete cDNA sequence was obtained and named as Sihsp83 (GenBank accession no.: KM077137). This was 2 496 bp in length and contained an opening reading frame (ORF) of 2 154 bp encoding 717 amino acid residues with a predicted molecular weight of 82.6 ku. Five conserved sequences of the HSP90 family exist in this amino acid sequence. The cytoplasmic localization signal was present in the C-terminal. Analysis of the genomic DNA sequence (GenBank accession no.: KM077138) demonstrated that the HSP83 gene does not contain introns. [Conclusion]  Full-length cDNA and genome sequences of the HSP83 gene from S. inferens were obtained, and do not contain introns. Sequence analysis confirms that HSP90 is very conservative.