[Objectives]  Cytochrome P450s are ubiquitous superfamily enzymes that play important roles in the metabolism of endogenous and exogenous compounds in insects. In order to provide a basis for the further study of the molecular properties and biological functions of the CYP408B1 and CYP409A1 genes in Locusta migratoria, we analyzed their expression patterns in different tissues and performed the prokaryotic expression of these two genes. [Methods]  Total RNA of different tissues from fifth-instar nymphs of L. migratoria was used to synthesize cDNA using MLV reverse transcriptase. Real-time PCR and RT-PCR were conducted to analyze the mRNA expression patterns of CYP408B1 and CYP409A1. In addition, the constructed expression plasmids pCW/CYP408B1 and pCW/CYP409A1 were co-expressed with pAC/CPR in E. coli BL21 (DE3), respectively. [Results]  CYP408B1 and CYP409A1 were expressed in the antenna, brain, optic lobe, subpharygeal ganglion, thoracic ganglia and accessory gland, while CYP408B1 had higher expression levels in the accessory gland by PCR. The prokaryotic expression results show that CYP409A1 and CPR (NADPH-cytochrome P450 reductase) were successfully expressed in inclusion bodies. Their molecular weights were approximately 58 ku and 77 ku, respectively. The recombinant CYP408B1 was not expressed in the E. coli expression system. [Conclusion]  The results reveal the expression patterns of CYP408B1 and CYP409A1 in different tissues of L. migratoria and confirm the expression of the recombinant CYP409A1 and CPR by a prokaryotic expression system. These results provide an experimental basis and basic data for further research on the detoxification of insecticides by cytochrome P450 genes in L. migratoria.