Molecular cloning and bioinformatics analysis of the Bemisia tabaci Mediterranean (MED) transient receptor potential (TRP) gene
Author of the article:LI Qian1, 2** Lü Zhi-Chuang2 LI Zi-Ling1 WAN Fang-Hao2***
Author's Workplace：1. College of Agriculture, Guangxi University, Nanning 530004, China; 2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Key Words： Bemisia tabaci, TRP channel, thermoreceptor, gene cloning
Abstract： [Objectives] Recently, researchers have paid more attention to the insect temperature sensing mechanism of which the transient receptor potential (TRP) is one of key components. Studying the temperature sensing mechanism will provide a theoretical basis for understanding the regulatory mechanism involved in temperature adaptation. TRP plays important roles in perceiving temperature, such as avoiding extreme temperatures and choosing the optimum temperature. [Methods] In the present study, we used 3′RACE and 5′RACE to isolate the TRP gene cDNA sequence from the Bemisia tabaci (Gennadius) Mediterranean (MED) cryptic species, and bioinformatics to characterize the TRP genes molecular structure and analyze its phylogenetic relationships. [Results] The TRP gene of B. tabaci MED, named BtTRP, had an open reading frame of 3 501 bp, encoding 1 166 amino acids. The encoded products included 6 transmembrane structures, which are one of the features of transient receptor ion channels. Bemisia tabaci MED BtTRP displayed 75% and 71% similarity with that of Acyrthosiphon pisum (Harris) and Bombus terrestris (Linnaeus), respectively. [Conclusion] These results provide basic information for further study of the BtTRP gene function underlying B. tabaci MED temperature perception, and provide a theoretical basis for understanding the temperature perception of insects and their adaptability.