In this study, microsatellite primers were designed based on the sequences of migratory locust in Gen Bank and tested for their workability. The results showed that only one of three pairs of primer was workable and could be used to amply one microsatellite locus. The sequence of this locus contained repeat units of (TG)_n and (GC)_n, meaning a discontinuous microsatellite. At this locus there were 14 alleles. The allele number, size and frequency for different locust populations were much different. Chi-squared tests for the genotype frequencies at this locus were erformed. The average expected and observed heterozygosity for this locus was 0883 6 and 0.457 8, respectively, indicating the genotype frequency of this loci deviated from HardyWeinberge equilibrium (χ²=733.12, P=0.0000). The highly polymorphism of this microsatellite locus made it suitable to sever as a good molecular genetic marker to analyze genetic difference of geographical populations of migratory locust.