Haemolymph is one of the most important materials for the physiological and biochemical study of insects. In this paper various methods for collecting insect haemolymph from different insects are analyzed and summarized. Insect preparation before haemolymph collection and pretreatment of the collected haemolymph are introduced. Meanwhile, based on the relative research in our laboratory, problems and experience during collecting haemolymph are expounded in order to provide an effective way for the experiments involving haemolymph collection.