[Objectives]  To clarify the effect of different foods on the fungal community of adult male and female Nasonia vitripennis (Walker). [Methods]  We cultured fungi from N. vitripennis after feeding them with different concentration and dilution plate techniques, and different media, and quantified the results using both morphological and molecular (ITS gene) methods. [Results]  Forty-nine different fungal species were isolated from male and female adults of N. vitripennis before and after feeding. These were classified into 4 classes (Dothideomycetes, Eurotiomycetes, Leotiomycetes, Ascomycetes) and 10 genera of Ascomycota. The predominant genera were Alternaria, Cladosporium and Penicillium. [Conclusion]  Males had a greater number of fungal species than females, and more fungi were cultured after feeding than before. These results indicate that food and the gender are important factors affecting fungi that can be cultured from N. vitripennis.