[Objectives]  Chemosensory proteins (CSPs) are a kind of soluble small protein that is widespread in the antenna, and other chemical sensory organs, of insects. CSPs participate in the chemical behavior of insects and play a key role in chemoreception. We investigated the spatiotemporal expression levels, and explored the functions, of three CSPs; Amel-CSP1, Amel-CSP2 and Amel-CSP6, in adult Apis mellifera ligustica workers. [Methods]  Expression levels of Amel-CSP1, Amel-CSP2 and Amel-CSP6 in different parts of nurse and forager bees, and in the antennae of 1, 6 12, 18 and 28-day-old workers, were quantified using RT-qPCR. [Results]  (1) Amel-CSP1 was expressed to a high degree in the antennae and heads of adult workers, and its expression in antennae was significantly higher than in others body parts (P<0.05). Amel-CSP2 was expressed more highly in the antennae of nurses (P < 0.05), whereas in foragers, expression of this gene was significantly higher in the head than in others organs (P < 0.05). Amel-CSP6 was expressed most highly in the heads of nurses and foragers and also relatively highly in the antennae and thorax. (2) Expression of Amel-CSP1 increased with age, being significantly more highly expressed in 28-day-old workers (foragers) than in young workers (P < 0.05). Amel-CSP2 was significantly more highly expressed in 1 and 12-day-old workers than in those of other ages (P < 0.05). Expression of Amel-CSP6 was significantly lower in 28-day-old workers than in those of other ages (P < 0.05). [Conclusion]  The expression levels of Amel-CSP1、Amel-CSP2 and Amel-CSP6 all showed a high degree of spatiotemporal specificity in adult Apis mellifera ligustica workers. These results provide a theoretical basis for further exploration of the physiological functions of Amel-CSP1、Amel-CSP2 and Amel-CSP6, in A. m. ligustica.