Abstract  [Aim]  This study aims to investigate the effects of heat shock protein P-Hsp70 gene expression on the longevity and fecundity of adult 14-spotted ladybugs, Propylaea quatuordecimpunctata, under different temperatures. [Methods] Microinjection delivery of dsRNA was used to interfere with the P-Hsp70 gene of adult P. quatuordecimpunctata. qRT-PCR was used to calculate the interference efficiency of the P-Hsp70 gene. The adults were then exposed to different temperatures (32, 35, and 38 ℃) to determine the effect of temperature on their survival and reproduction following P-Hsp70 gene interference. [Results]  Our results demonstrate that microinjection delivery of dsRNA can effectively inhibit the expression of the P-Hsp70 gene in adult P. quatuordecimpunctata. The survival rate, longevity, and fecundity of adult P. quatuordecimpunctata treated with microinjection ds-P-Hsp70 were significantly lower than the control group (without injection of dsRNA) under all temperatures. At 32, 35, and 38 ℃, the survival rate of adults decreased by 15.5%, 16.7%, and 56.8%, respectively. Additionally, the average adult longevity was reduced by 40.6%, 51.2%, and 65.8%, respectively. At 32  and 35 ℃, the fecundity of adults decreased by 37.5% and 100.0%, respectively. At 38 ℃, the adults failed to lay eggs with or without dsRNA. [Conclusion]  Our findings indicate that the heat shock protein P-Hsp70 gene plays a crucial role in high temperature stress resistance in adult P. quatuordecimpunctata.