Abstract  [Aim]  To develop a new screening system for Riptortus pedestris effectors that target different plant resistance related pathways using luciferase as a reporting gene, and apply these it to the study of R. pedestris effectors. [Methods]  Four resistance-related soybean genes that are highly upregulated after R. pedestris damage to soybean plants, and that are involved in different soybean signaling pathways, were selected after comparative analysis of two soybean transcriptomes from R. pedestris infested, and non-infested, leaves. The four genes were, Gm2, which participates in the isoflavone metabolism, Gm3, which is involved in the auxin and glucosinolate metabolism, Gm6, which is related to immune signal recognition and Gm8, which is involved in the biosynthesis of jasmonic acids. All promoter sequences of these four genes were amplified with PCR, then inserted into the pGreen 35s-LUC vector. Successfully constructed vectors were transferred to Escherichia coli, and then transiently expressed in the leaves of Nicotiana benthamiana using the A. tumefaciens infiltration method to establish reporting systems. After each R. pedestris effector had been transiently expressed in the leaves of N. benthamiana for 12 h, the reporting systems were expressed in the same area of each leaf. The function of each effector was determined by observing the impact of each gene on the expression of luciferase. [Results]  Agarose gel electrophoresis and gene sequencing confirmed that four promotors of soybean resistance-related genes were successfully amplified using PCR. The luminous intensity of the luciferase reporter gene and an enzyme activity detection assay using Microplate Reader and Western Blot, demonstrated that all four promoters were successfully expressed in the leaves of N. benthamiana. The results of a feeding assay, mechanical plant damage, and treatment with methyl jasmonate (MeJA), confirm that this new screening system is reliable. The system was subsequently used to screen R. pedestris effectors and indicates that nine R. pedestris effectors have different regulatory functions on the expression of the four reporter genes. [Conclusion]  A new screening system for R. pedestris effectors, using luciferase as reporter, was successfully developed and used to select R. pedestris effectors. This new screening system is easy to operate, efficient and fast. It provides a novel approach for research on other insect effector genes.