Effects of a decoction of Aspongopus chinensis on the proliferation, migration, apoptosis, and related mRNA expression, of breast cancer cells in vitro
Author of the article:TIAN Ying1, 2** TAN Jun2 MA Sheng-Feng3 ZHAO Shuai1 CAI Ren-Lian1, 2 GUO Jian-Jun1***
Author's Workplace：1. Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Institute of Entomology, Guizhou University, Guiyang 550025, China; 2. College of Basic Medicine, Zunyi Medical University, Zunyi 563000, China; 3. Gong’an County Agriculture and Rural Science and Technology Service Center, Jingzhou 434399, China
Key Words： Aspongopus chinensis; decoction; breast cancer; proliferation; migration; apoptosis; mechanism
Abstract：
[Aim] To investigate
the effects of a decoction of Aspongopus chinensis (ACD) on the proliferation,
migration, cell cycle and apoptosis, of MDA-MB-453 and
HCC1937 breast cancer cells. [Methods] The CCK-8 method was conducted to detect the
effect of ACD on the proliferation of breast cancer cells in vitro and a
cell wound healing assay was used to investigate the effect of ACD
on their migratory ability. The cell cycle
distribution and apoptosis rate were detected using flow cytometry. The mRNA
expression of Caspase-3, Caspase-8, Bax and Bcl-2 (Genes
associated with apoptosis), MMP-2 and MMP-9 (Genes involved in
cell migration), CDK-1, CDK-2, CyclinA1 and CyclinB1 (Cell cycle
related genes) were detected using RT-PCR. [Results] ACD inhibited the proliferation and migration
of breast cancer cells in vitro by down-regulating MMP-2 and MMP-9.
It also induced apoptosis by up-regulating Caspase-3, Caspase-8 and Bax and by
down-regulating Bcl-2. It blocked MDA-MB-453 cells in the G1 phase, and HCC1937
cells in the G2 phase, possibly by down-regulating CDK-1,
CyclinA1 and CyclinB1. [Conclusion] ACD can inhibit the proliferation and
migration of MDA-MB-453 and HCC1937 breast cancer cells, regulate the cell cycle of these cells and induce
their apoptosis in vitro.