The geometric morphometrics of eight Euchirinae species in China
Author of the article:YIN Fan1, 2** TONG Yi-Jie2 LU Yuan-Yuan2 TIAN Zhe-Hao1, 2 BAI Ming2***
Author's Workplace:1. School of Agriculture, Ningxia University, Yinchuan 750021, China; 2. Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
Key Words:Euchirinae; geometric morphometrics; character screening; protibia; pronotum; elytron
Abstract:
[Aim] Euchirinae
is a subfamily of Scarabaeidae, or scarab beetles. At present, two genera and
eight species of Euchirinae have been found in China. These include six species
from the genus Cheirotonus and two species from the genus Propomacrus,
all of which have been listed as national second-class protected animals in the
2021 edition of the ‘List of wild animals under priority conservation’. The
species of the subfamily Euchirinae distributed in China are rare, and the
biological information of the subfamily Euchirinae has been difficult to obtain
for a long time, resulting in slow progress in the collection and taxonomic
research of systematic morphological information between groups. The
identification of closely related species is also very difficult, which poses
certain obstacles to the inspection work of customs and public security
departments. Based on geometric morphometrics, our study quantitatively
analyzed the morphological characteristics of male and female Euchirinae
samples in China, revealing the morphological differences among 8 species of
Euchirinae distributed in China. We also screened additional morphological
characteristics for classification and identification. This study offers
innovative approaches for improving the customs inspection process. [Methods] Based on geometric morphometrics, we compared
the outer contours of the protibia of eight species of Euchirinae, and the
morphology of the left outer contours of the pronotum and elytron in both males
and females, using principal component analysis (PCA), canonical variance
analysis (CVA), and discriminant function analysis (DFA). The morphological
differences between male and female individuals were analyzed, and the
identification features recorded. [Results] Based on the PCA results, we found
that the morphological changes of the protibia in the intergroup segment of
Euchirinae were concentrated in the curvature of the spines and tibia. Whereas
variation in the pronotum resulted from the expansion of the posterior horn and
the reduction of the aspect ratio. Finally, we found elytron variation was due
to extension of the shoulder angle, the interaction of the elytron near the
scutellum, and a reduction in the aspect ratio. The CVA and DFA results showed
a significant difference in the morphology of the male protibia between
species. Of the test groups, 93% (26/28) achieved a 100% discriminant accuracy
rate between test groups. Finally, CVA analysis showed there was no
intersection between the elytron morphology of the eight female long-armed
scarabs. [Conclusion] The morphological characteristics of the male
protibia differed significantly between Euchirinae species. Furthermore, the
elytrons of female samples exhibited stronger interspecific differentiation.
Geometric morphometrics can facilitate the identification of morphological
differences between Euchirinae species in China and be used for interspecific
identification.