A method for rapidly determining the sex of adult and pupae of Thosea sinensis (Lepidoptera: Limacodidae)
Author of the article:FU Shu1, 2** HU Gui-Ping3 YANG Bin-Huang1, 2 CAO Hong-Mei3 XIE Xiao-Qun3 HE Wang-Xing3 XUE Fa
Author's Workplace:1. College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou 362000, China; 2. Key Laboratory of Inshore Resources Biotechnology, Fujian Province University, Quanzhou 362000, China; 3. Jiangxi Cash Crops Research Institute, Nanchang 330046, China; 4. Institute of Entomology, Jiangxi Agricultural University, Nanchang 330045, China; 5. College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Key Words: Thosea sinensis; morphological character; pupa; adult; sex identification
Abstract:
[Aim] Thosea sinensis is an important pest of tea. A simple quick and accurate
method was developed to sex pupae and adults of T. sinensis to provide more accurate data for field population
monitoring and prediction of this insect. [Methods] The morphological characteristics of pupae
and adult of T. sinensis were
observed and compared. Adult genital types were used to validate the results of
sexing. [Results] The reproductive pore in female pupae is
located on the 8th ventral segment and the oviposition hole is on the 9th
ventral segment. The surroundings of these two holes are flat with no
protrusions. The reproductive pore is on the 9th ventral segment and is
characterized by a longitudinal crack shape. In male pupae there are
semi-circular tumor-like protrusions on both sides of the reproductive pore. In
female adults, the antennae are filamentous, and the end of the abdomen is
cylindrical. Pressing the abdomen gently reveals that the oviposition valve is
flat and oval. The antennae of male adults are comb-toothed, and the length of
antennae is significantly shorter than that of adult females. In adult males,
the end of the abdomen is thinner, and their reproductive organs feature a
pincer-shaped grasper. The method can quickly identify the sex of adult and
pupae of T. sinensis with 100% accuracy. [Conclusion] Male and female T. sinensis pupae can
be accurately distinguished by determining the position and shape of the
reproductive pore. The sex of the adults can be accurately distinguished by
observing the antennae or the genital structure by lightly pressing the
abdomen.