[Aim]  The family Asilidae belongs to Brachycera, a suborder of the Diptera. All insects in this family are carnivorous, the adults preying on insects such as wasps, locusts, leafhoppers and flower flies. This study aims to better understand the mitochondrial genome characteristics and phylogenetic relationships of the Asilidae. [Methods]  This paper presents the results of the first omics analysis and phylogenetic discussion based on the mitochondrial genome sequences of 16 species of this group. [Results]  The mitochondrial genome of the Asilidae includes 22 tRNAs, 13 protein coding genes, 2 rRNAs, and 1 control region. The gene sequence is consistent with that of Drosophila melanogaster, and no gene rearrangement has been found. Analysis of the nucleotide composition shows that AT content is much higher than GC content, indicating a clear AT bias. The starting codons of nine genes, including atp6, atp8, cox2, cox3, cytb, nad2, nad3, nad4l, and nad6 are all ATN, whereas cox1, nad1, nad4, and nad5 not only have ATN but also TCG and GTG. The end codons are very similar, mostly TAA, TAG, but also the incomplete termination codons T and TA. Relative synonymous codon usage shows that the UUA codon of Leu2 was the most frequent, whereas the GCG codon of Ala and the ACG codon of Thr were the least common. The evolutionary rate of the 13 protein coding genes can be ranked as follows: atp8 > nad6 > nad4 > nad5 > nad2 > nad4l > nad1 > nad3 > atp6 > cox2 > cox3 > cytb > cox1. [Conclusion]  A phylogenetic analysis based on the PCGs dataset has confirmed the monophyly of the family Asilidae, the subfamily Asilinae, and the subfamily Leptogastrinae. This study provides a scientific basis for in-depth understanding of the biological characteristics, origin, and evolution, of insectivorous insects.