Spatio-temporal expression profile and molecular properties of the Apis cerana cerana PP2A gene
Author of the article:KANG Jing1** CHEN Ying1** SUN Kai-Yue1 WANG Yong-Jie1 ZANG He1, 2, 3 FENG Rui-Rong4 FU Zhong--Min1 CHEN Da-Fu1, 2, 3 QIU Jian-Feng1, 2, 3*** GUO Rui1, 2, 3***
Author's Workplace：1. College of Bee Science and Biomedicine, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2. National & Local United Engineering Laboratory of Natural Biotoxin, Fuzhou 350002, China; 3. Apitherapy Research Institute of Fujian Agriculture and Forestry University, Fuzhou 350002, China; 4. Fujian Vocational College of Agriculture, Fuzhou 350000, China
Key Words：Apis cerana cerana; protein phosphatase 2A; spatio-temporal expression profile; molecular features of proteins
Abstract：
[Aim] To determine the spatio-temporal
expression profile of the Apis cerana cerana protein phosphatase 2A (PP2A) gene, analyze its molecular
characteristics and physical and chemical properties in order to provide a
reference for further research. [Methods] The relative expression of AcPP2A in different tissues, including the antennae, midgut, brain, venom gland,
cuticle, hypopharyngeal gland, and fat body, were determined using RT-qPCR, as were its relative expression in eggs,
larvae, pre-pupae, pupae and adult workers of different ages. Using the ORF
tool on the NCBI website, Protparam, ProtScale, and SWISS-model software on the
Expasy website, SignalP 4.1 Server, NetPhos 3.1 Server, TMHMM, and SOPMA, and
other software related software Predicting molecular features of the AcPP2A
protein and the physicochemical properties, identify conserved motifs and
structural domains of PP2A in A. c. cerana and other species, and
construct a phylogenetic tree. [Results] AcPP2A contains 5 174
nucleotides, encodes 475 amino acids and has a protein molecular weight of
about 54.78 kD. It has a lipid solubility coefficient of 78.80, an isoelectric
point of 5.97, a molecular formula of C2411H3781N671O742S23,
and an average hydrophilic coefficient of ﹣0.521. AcPP2A
does not have a transmembrane domain or signaling peptide. PP2A of A. c.
cerana, Melipona quadrifasciata, Apis mellifera, Bombus
impatiens, Temnothorax curvispinosus, Megachile rotundata, B.
pyrosoma, Frieseomelitta varia, Acromyrmex echinatior, Habropoda
laboriosa and Lingula anatina, all have the same six conserved
motifs (Motif1, Motif2, Motif3, Motif4, Motif5 and Motif6) and one identical
domain (the CDC55 superfamily). PP2A of A. c. cerana and T.
curvispinosus cluster on the same branch of the phylogenetic tree. [Conclusion] AcPP2A appears to be an acidic, hydrophilic,
non-transmembrane, highly conserved, intracellular protein. Its expression
varies widely across different tissues and developmental stages, but it may
play an important role in the development of the venom gland and eggs.