[Aim]  This study aims to perform bioinformatic analysis of the ubiquitin-conjugating enzyme E2-22 kD (AmUBCD4) in Apis mellifera and determine its expression patterns in various tissues and developmental stages of worker bees, providing a basis for exploring its functional roles. Bioinformatic tools were employed to analyze the physicochemical properties, molecular structures (including secondary and tertiary structures), and protein interaction network of AmUBCD4. Meanwhile, a comparative study was conducted on the conserved motifs and domains of UBCD4 between A. mellifera and other insects. A comparison of conserved motifs and structural domains in UBCD4 in A. mellifera and other insects was also conducted. RT-qPCR was used to evaluate the relative expression of AmUBCD4 in different worker tissues, including the brain, hypopharyngeal gland, midgut, antenna, venom gland, cuticle, and fat body, as well as in different developmental stages, including eggs, larvae, pre-pupae, and pupae. [Results]  AmUBCD4 consists of 199 amino acids, with a molecular formula of C₁₀₁₀H₁₅₈₄N₂₇₀O₂₉₈S₇. Its molecular weight is approximately 22.50 kD, and the theoretical isoelectric point is 5.31. The instability index of AmUBCD4 is 45.22, the half-life is 30 h, the lipid coefficient is 88.29, and the average hydrophilicity coefficient is﹣0.303. Additionally, AmUBCD4 contains 25 phosphorylation sites, while it lacks a transmembrane domain and a signal peptide. AmUBCD4 putatively interacts with 10 proteins, including Uba1 and RpL40. UBCD4 has 5 conserved motifs common to A. mellifera and other nine insects. Both the UBA_Ⅱ_E2_UBCD4 and the UBCc structural domains are present in UBCD4 in A. mellifera, Megachile rotundata, Nasonia vitripennis, Polistes dominula, A. cerana, Bombus terrestris, A. florea and Bombyx mori. AmUBCD4 was differentially expressed in the antennae, brain, midgut, venom gland, fat body, cuticle and hypopharyngeal gland, with the highest expression in the cuticle and the lowest in the midgut. With respect to developmental stages, expression was highest in eggs, and progressively decreased in subsequent developmental stages, being lowest in 12-day-old pupae. [Conclusion]  AmUBCD4 is a potential hydrophilic, intracellular protein that is most highly expressed in the cuticle and hypopharyngeal gland, and in egg stage, of A. mellifera workers.