[Aim]  To identify and conduct a bioinformatic analysis of the visual gene of Phyllotreta striolata and thereby provide a basis for further study of phototaxis in this species. [Methods]  The transcriptome was analyzed and annotated by cDNA library construction and PacBio sequencing. BioEdit, ExPASY, TMHMM, AlphaFold, and other software, were then used to analyze and identify the physicochemical properties of the P. striolata opsin genes PstrOpsin-LW, PstrOpsin-UV1, PstrOpsin-UV2 and Pstr-Pteropsin, and predict their protein structure and functional sites. Sequence analysis and phylogenetic tree construction were performed using MEGA 7.0 software. Finally, real-time fluorescent quantitative PCR was used to analyze the spatio-temporal expression, and expression in each gender, of these 4 opsin genes. [Results]  Four genes were identified by the transcriptome sequencing, namely long-wave sensitive opsin PstrOpsin-LW, ultraviolet light-sensitive opin PstrOpsin-UV1 and PstrOpsin-UV2, and non-visual opin Pstr-Pteropsin, all of which belonged to the typical structure of G-protein-coupled receptors. The expression levels of PstrOpsin-LW, PstrOpsin-UV1 and PstrOpsin-UV2 were significantly higher in adults than in pupae and larvae (P<0.05), but there was no significant difference in the expression of Pstr-Pteropsin in different instars (P>0.05). The expression of the four genes in the head of adults was significantly higher than in the body (P<0.05), and PstrOpsin-UV1 and Pstrosin-UV2 were highly expressed in the head of male and female adults, respectively. [Conclusion]  Four retinopsin genes were identified and their molecular characteristics were clarified. Differences in the expression of these genes during growth and development were revealed, which is useful information for the prevention and control of P. striolata.