Determination of the supercooling and freezing points of different developmental stages of Atractomorpha sinensis (Orthoptera: Pyrgomorphidae)
Author of the article:LI Wen-Bo1** Muhammad·Naeem1 LUO Chang-Xin1 CUI Juan2 WANG Qing-Song3 CHEN Huan-Huan1***
Author's Workplace：1. College of Biological Resource and Food Engineering, Qujing Normal University, Qujing 655011, China; 2. College of Agriculture, Jilin Agricultural Science and Technology College, Jilin 132101, China; 3. Citrus Research Institute of Southwest University, Chongqing 400712, China
Key Words：Atractomorpha sinensis; supercooling point; freezing point; cold hardiness
Abstract：
[Aim] The
purpose of this study was to clarify the cold tolerance of different insect
states of Atractomorpha sinensis, and to provide a theoretical basis for
predicting the overwintering situation of the pest in the field and the
occurrence dynamics in the next year in Qujing area. [Methods] The supercooling point tester was used to
determine the stages (instars) of A.
sinensis. [Results] The
supercooling and freezing points of eggs were the lowest, -18.60 and -9.13 ℃, respectively. The supercooling
point of 5th instar nymphs, and the freezing point of adult females, were the
highest; -11.50 and -6.97 ℃, respectively. Variation in the supercooling and freezing points of individuals in the same stage of
development was normally distributed. The supercooling and freezing point of
adult males were -13.00 and -7.88 ℃, respectively, lower than those of
adult females, which were 12.03 and -6.97 ℃, respectively. [Conclusion] The supercooling point of A. sinensis eggs was the lowest among the developmental
stages examined, whereas that of 5th instar nymphs was the highest. These results provide
basic data on the overwintering mechanism of A. sinensis, and predict areas where this species could either
be found or establish.