Determining the baseline susceptibility of Maruca vitrata (Lepidoptera: Crambidae) larvae to commonly used insecticides
Author of the article:ZHOU Zhe-Qing1, 2** ZHOU Li-Lin1 WANG Yong1 YANG Fan1 YANG Shao-Li1 CAI Xiang1 LUO Hai-Bo1 SI Sheng-Yun1 WANG Pan1***
Author's Workplace:1. Institute of Vegetables, Wuhan Academy of Agricultural Sciences, Wuhan 430345, China; 2. College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, China
Key Words: Maruca vitrata; insecticides; diet surface overlay bioassay; diet incorporation bioassay; susceptible baseline
Abstract:
[Aim] Two bioassay methods were used to
evaluate the toxicities of different types of insecticides against Maruca
vitrata larvae, and to establish the relative susceptibility baselines of M.
vitrata to commonly used insecticides, in order to provide reference for
systematically resistance monitoring and management of M. vitrata in
China. [Methods] The
susceptibility of the 3rd instar larvae of M. vitrata to eight commonly
used insecticides (chlorantraniliprole, cyantraniliprole, indoxacarb, emamectin
benzoate, abamectin, spinetoram, lufenuron, broflanilide) belonging to five
insecticide categories were determined by diet surface overlay bioassay method
and diet incorporation bioassay method, respectively. Based on the
bioassay results, the relative susceptible baselines of M. vitrata to
these eight insecticides were established. [Results] The LC50 values of the above
eight insecticides to M. vitrata larvae were 0.007-1.413 μg/cm2 by diet surface overlay bioassay, and 0.025-4.178 mg/L by diet incorporation
bioassay, respectively. Compared with the diet surface overlay bioassay method,
the bioassay data obtained in diet incorporation bioassay method had smaller chi-square
value and larger P-value. At the same time, M. vitrata populations showed higher genetic purity of the population in the diet
incorporation bioassay method than in the diet surface overlay bioassay
method, and also showed the highest repeatability of bioassay results. So it is
the recommended bioassay method of these commonly used insecticides against M.
vitrata larvae (except indocarb). [Conclusion] The relative susceptibility baselines of M.
vitrata larvae to eight commonly used insecticides have been established
using two different bioassay methods respectively, providing reference for the
resistance monitoring and chemical control of M. vitrata in China.