Screening and optimization of artificial diets for Euzophera pyriella larvae
Author of the article:HE Xiao-Ling1** ZHANG Da-Wei1, 2 GUO Huan1, 3 WANG Hao-Yu1, 3 LU Yan-Hui1***
Author's Workplace:1. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 2. Institute of Plant Protection, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China; 3. College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, China
Key Words: Euzophera pyriella; artificial diets; life table of population; larval survival rate
Abstract:
[Aim] To compare the larval survival and development, adult longevity
and reproduction, population life table parameters, of Euzophera pyriella larvae
reared on three different artificial diets, and the relative cost effectiveness
of each diet. [Methods] E.
pyriella larvae were randomly assigned to one of three potential
artificial diet treatment groups after which the survival, development, adult
longevity, reproduction and life table parameters of each group were compared.
These data were then used to assess the cost effectiveness of rearing larvae on
each diet. [Results] Larvae were
able to complete their life cycle on all three artificial diets. Compared to
the previously developed A and B diets, the newly developed diet C
significantly improved the larval development rate, survival rate, pupal weight
and egg hatching rate (P<0.05). Larval
survival increased from 75.00% and 60.00% on diets A and B, to 91.67% on diet
C. Furthermore, the population parameters of E. pyriella raised on
diet C, including the net reproductive rate (R0), the intrinsic rate of
increase (r), and the finite rate of increase (λ), were also
significantly higher (P<0.05). The intrinsic rate of increase (r) increased from
0.057 and 0.059 on diets A and B, to 0.079 on diet C. The estimated cost of
successfully rearing one pupa on diet C was 0.10 CNY, representing a saving of
37.50% and 60.00% relative to diets A and B, respectively. [Conclusion] Based on rearing efficiency and cost,
artificial diet C is superior to diets A and B for the multi-generational
rearing of E. pyriella under laboratory conditions.