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Issue:ISSN 2095-1353
           CN 11-6020/Q
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Your Position :Home->Past Journals Catalog->2025年62 No.6

The complete sequence characteristics and phylogeny of the mitochondrial genome of Tapiena bivittata
Author of the article:LIU Fei** ZHANG Lan-Yi HUA Wen-Ping LI Jia YUAN Zhi-Min WANG Li-Zhi***
Author's Workplace:Key Laboratory of Qinling Medicinal and Edible Biological Resources Conservation and Utilization, College of Biology, Food and Chemistry, Shaanxi Xueqian Normal University, Xi’an 710061, China
Key Words:Tapiena bivittata; mitochondrial genome; Phaneropterinae; Tettigoniidae; Orthoptera; phylogeny
Abstract:

 [Aim]  To describe the mitochondrial genome characteristics of Tapiena bivittata, and clarify its phylogenetic relationships with other members of the Phaneropterinae. [Methods]  The complete mitochondrial genome of T. bivittata was first sequenced using next-generation sequencing technology, then assembled, annotated, and analyzed. Based on the mitochondrial genome sequence of T. bivittata and the published mitochondrial genomes of 21 Phaneropterinae species, 13 protein-coding genes and 2 rRNA genes were extracted. Bayesian inference was used to reconstruct phylogenetic relationships within the subfamily. [Results]  The complete mitochondrial genome length of T. bivittata is 16 414 bp, and is comprised of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 1 control region. The gene arrangement is compact and consistent with that of the ancestral gene. The A+T content of the mitochondrial genome is 69.7%. With the exception of COX1 and ND1, which use TTG as the start codon, the remainder of the 13 protein-coding genes use ATN. All genes use TAG or TAA as termination signals, except for COX2, COX3, ND5, and ND4, which use the incomplete stop codon T. With the exception of tRNASer(AGN), all other 22 tRNAs have a typical cloverleaf structure. [Conclusion]  The complete mitochondrial genome sequence of T. bivittate was successfully obtained. A Bayesian phylogenetic tree indicates that T. bivittata is most closely related to three species of the genus Ruidocollaris: Ruidocollaris convexipennis, Ruidocollaris obscura, and Ruidocollaris sinensis.

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