The complete sequence characteristics and phylogeny of the mitochondrial genome of Tapiena bivittata
Author of the article:LIU Fei** ZHANG Lan-Yi HUA Wen-Ping LI Jia YUAN Zhi-Min WANG Li-Zhi***
Author's Workplace:Key Laboratory of Qinling Medicinal and Edible Biological Resources Conservation and Utilization, College of Biology, Food and Chemistry, Shaanxi Xueqian Normal University, Xi’an 710061, China
Key Words:Tapiena bivittata; mitochondrial genome; Phaneropterinae; Tettigoniidae; Orthoptera; phylogeny
Abstract:
[Aim] To describe the mitochondrial genome
characteristics of Tapiena bivittata, and clarify its phylogenetic
relationships with other members of the Phaneropterinae. [Methods] The complete mitochondrial genome
of T. bivittata was first sequenced using next-generation sequencing
technology, then assembled, annotated, and analyzed. Based on the mitochondrial
genome sequence of T. bivittata and the published mitochondrial genomes
of 21 Phaneropterinae species, 13 protein-coding genes and 2 rRNA genes were extracted.
Bayesian inference was used to reconstruct phylogenetic relationships within
the subfamily. [Results] The complete
mitochondrial genome length of T. bivittata is 16 414 bp, and is
comprised of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 1
control region. The gene arrangement is compact and consistent with that of the
ancestral gene. The A+T content of the mitochondrial genome is 69.7%. With the exception
of COX1 and ND1, which use TTG as the start codon, the remainder
of the 13 protein-coding genes use ATN. All genes use TAG or TAA as termination
signals, except for COX2, COX3, ND5, and ND4, which
use the incomplete stop codon T. With the exception of tRNASer(AGN),
all other 22 tRNAs have a typical cloverleaf structure. [Conclusion] The complete
mitochondrial genome sequence of T. bivittate was successfully obtained.
A Bayesian phylogenetic tree indicates that T. bivittata is most closely
related to three species of the genus Ruidocollaris: Ruidocollaris
convexipennis, Ruidocollaris obscura, and Ruidocollaris sinensis.