Identification of Bactrocera tsuneonis and Bactrocera minax in the field
Author of the article:MA Bao-Sheng1** FENG Tai-Yang1 LI Lai-Wen1 YANG Yi-Juan2 YANG Jin-Rong3 CHEN Min2*** DONG Wen
Author's Workplace:1. State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan,College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China; 2. Plant Protection and Quarantine Station of Zhaotong City, Zhaotong 657000, China; 3. Plant Protection and Quarantine Station of Yanjin County, Yanjin 657000, China
Key Words:Bactrocera tsuneonis; Bactrocera minax; morphological differences; ripid identification; field monitoring
Abstract:
[Aim] To
develop a method for the rapid visual identification of two citrus fruit fly
species, Bactrocera tsuneonis and Bactrocera minax,
under field conditions. [Methods] Morphological
features of both species were examined using visual checks and smartphone
photos to identify differences specific to each species. [Results]
Males could be distinguished by two tergal features: (1) Transverse band
coloration on abdominal segments 4-5 (black in B. tsuneonis vs. light brown in B.
minax), and (2) The presence or absence of paired lateral depressions on
segment 4 (absent in B. tsuneonis,
present in B. minax). These two
criteria achieved identification accuracies of 94.59% and 100.00%,
respectively. Furthermore, morphological examination using a smartphone-mounted
macro lens revealed distinct differences in the coaxial setation between the
two species. B. tsuneonis has dense
clusters of dark setae on both fore and mid-leg coxae, whereas these structures
were conspicuously absent in B. minax.
Females were distinguished by comparing the basal thickened oviscape segment
with the distal slender portion incorporating the eversible membrane. In B. tsuneonis the basal portion is longer
than the distal part, whereas in B. minax the reverse is true. This criterion distinguished females of these species with
96.77% accuracy. [Conclusion] The morphological criteria identified
in this study can rapidly, and
reliably, identify B. tsuneonis and B. minax in the field. Males of
these species can be distinguished by first observing the coloration patterns
of the transverse bands on the fourth and fifth abdominal segments, followed by
verifying the presence or absence of lateral depressions on the fourth
abdominal segment. Distinguishing females of these species requires two steps;
comparing the relative lengths of the basal portion of the oviscape to the
combined length of the distal oviscape and eversible membrane, followed by
microscopic examination of ovipositor apex morphology using smartphone macro
photography.