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Issue:ISSN 2095-1353
           CN 11-6020/Q
Director:Chinese Academy of Sciences
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Your Position :Home->Past Journals Catalog->2025年62 No.6

Identification of Bactrocera tsuneonis and Bactrocera minax in the field
Author of the article:MA Bao-Sheng1** FENG Tai-Yang1 LI Lai-Wen1 YANG Yi-Juan2 YANG Jin-Rong3 CHEN Min2*** DONG Wen
Author's Workplace:1. State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan,College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China; 2. Plant Protection and Quarantine Station of Zhaotong City, Zhaotong 657000, China; 3. Plant Protection and Quarantine Station of Yanjin County, Yanjin 657000, China
Key Words:Bactrocera tsuneonis; Bactrocera minax; morphological differences; ripid identification; field monitoring
Abstract:

[Aim]  To develop a method for the rapid visual identification of two citrus fruit fly species, Bactrocera tsuneonis and Bactrocera minax, under field conditions. [Methods] ‌ Morphological features of both species were examined using visual checks and smartphone photos to identify differences specific to each species. [Results] ‌ Males could be distinguished by two tergal features: (1) Transverse band coloration on abdominal segments 4-5 (black in B. tsuneonis vs. light brown in B. minax), and (2) The presence or absence of paired lateral depressions on segment 4 (absent in B. tsuneonis, present in B. minax). These two criteria achieved identification accuracies of 94.59% and 100.00%, respectively. Furthermore, morphological examination using a smartphone-mounted macro lens revealed distinct differences in the coaxial setation between the two species. B. tsuneonis has dense clusters of dark setae on both fore and mid-leg coxae, whereas these structures were conspicuously absent in B. minax. Females were distinguished by comparing the basal thickened oviscape segment with the distal slender portion incorporating the eversible membrane. In B. tsuneonis the basal portion is longer than the distal part, whereas in B. minax the reverse is true. This criterion distinguished females of these species with 96.77% accuracy.  [Conclusion]  The morphological criteria identified in this study can rapidly, and reliably, identify B. tsuneonis and B. minax in the field. Males of these species can be distinguished by first observing the coloration patterns of the transverse bands on the fourth and fifth abdominal segments, followed by verifying the presence or absence of lateral depressions on the fourth abdominal segment. Distinguishing females of these species requires two steps; comparing the relative lengths of the basal portion of the oviscape to the combined length of the distal oviscape and eversible membrane, followed by microscopic examination of ovipositor apex morphology using smartphone macro photography.

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