[Aim]  To analyze the molecular characteristics, conserved motifs, structural domains and phylogeny of the sphingosine-1-phosphate phosphatase 1 (AmSgpp1) gene of the western honeybee (Apis mellifera), and analyze the spatio-temporal expression pattern of AmSgpp1. [Methods]  The expression of AmSgpp1 was verified by PCR, and its molecular characterization, conserved motifs and phylogeny were analyzed using the relevant software. RT-qPCR was used to detect the relative expression of AmSgpp1 in the antennae, brain, hypopharyngeal glands, midgut, venom glands, epidermis and fat body, of adult worker bees, in eggs, 3-day-old larvae, 5-day-old larvae, 7-day-old pre-pupae, 9-day-old pre-pupae and 11-day-old pupae, and in 1, 2, 6, 8 and 12-day-old adult worker bees. [Results]  AmSgpp1 is authentically expressed in worker bees. Its CDS contains 1 302 nucleotides, encoding 433 amino acids. AmSGPP1 has an approximate molecular weight of 49.53 kD, a lipid solubility coefficient of 107.37, an isoelectric point of 8.59, and an average hydrophilic coefficient of 0.321. It can be simultaneously localized to the plasma membrane, cytoplasm and peroxisomes. AmSGPP1 contains seven transmembrane structural domains but no signaling peptide. The SGPP1 proteins of the A. mellifera and eight other species all contain one identical PAP2_SPPase1 structural domain, and 10 identical conserved motifs. AmSgpp1 is differentially expressed in seven adult worker tissues, with the highest expression in the fat body and midgut tissues, followed by the venom glands. AmSgpp1 is also differentially expressed in the egg, larvae, and pupal stages, with the highest expression in eggs and the lowest in 9-day-old pre-pupae. However, in adult worker bees, significantly higher expression was recorded in 12-day-old adults than in younger adults (P < 0.05). [Conclusion]  AmSGPP1 is a hydrophobic, intracellular, transmembrane protein. A. mellifera SGPP1 exhibits high conservation of motifs, domains, and amino acid sequence homology with the SGPP1 of other species. AmSgpp1 is differentially expressed across various tissues and developmental stages in A. mellifera.