[Aim]  Isolate and identify the fungus pathogenic that cause the death of Protaetia brevitarsis, and develop methods to reduce mortality during the artificial rearing of this species. [Methods]  A pathogenic fungal strain was isolated and purified using the plate culture method. Bioassays were used to determine the pathogenicity of the isolated strain, which was identified using morphological and molecular biological identification methods. The isolated strain was inoculated into feed provided to P. brevitarsis, and the effectiveness of 7 chemical fungicidesat suppressing fungal growth were evaluated. Three different treatments were set up: No fungi + fungicide, fungi + fungicide, and fungal infection + fungicide. The mortality and growth rate of the larvae under each treatment were measured and compared. [Results]  The pathogenic fungal strain was identified as Metarhizium anisopliae. Mortality rate of the 1st, 2nd and 3rd instars was 100.00%, 91.67% and 76.00%, respectively, 14 days after inoculation. The most effective treatment was 20% triadimefonemulsifiable concentrate, which reduced mortality tojust 12.22%±2.22%; a 62% reduction compared to the control. [Conclusion]  Adding 20% triadimefonemulsifiable concentrate to the diet of captive P. brevitarsis effectively preventedfungal infection, but was not very effective at reducing mortality onceinfection had occurred.