[Aim]  To investigate the effects of atropine, a secondary metabolite of Datura stramonium, on protective and detoxification enzyme activity in adult, female Frankliniella occidentalis. [Methods]  Using the membrane feeding method, the bioassay for toxicity of F. occidentalis female adults was conducted, and the 25%, 50%, and 75% lethal concentrations were calculated and designated as low, medium, and high concentrations, respectively. Female adults were then exposed to atropine solutions at these three concentrations via membrane feeding. The activity of three protective enzymes [catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD)], and three detoxification enzymes [glutathione S-transferase (GST), carboxylesterase (CarE), and cytochrome P450 (CYP450)], were then determined in each treatment group after 12, 24 and 48 h. [Results]  The activity of both protective and detoxification enzymes were significantly affected by atropine dosage. Protective enzyme (CAT, SOD, and POD) activity significantly increased in a concentration-dependent manner (CAT: P < 0.001; SOD: P < 0.001; POD: P < 0.001), with peak activity observed at 48 h. Notably, increases in SOD and POD activity were most pronounced in the high concentration treatment group. With respect to detoxification enzymes, GST and CYP450 activity initially increased, then decreased, peaking at 12 and 24 h, respectively. CarE activity was significantly enhanced only in the low-concentration group at 12 h (P < 0.001), but was continuously inhibited in the medium and high concentration groups. [Conclusion]  The activity of protective and detoxification enzymes in adult female F. occidentalis were significantly affected by atropine stress, which indicates that females of this species adapt to the effects of this plant secondary metabolite by regulating their protective and detoxification enzyme activity.