Effects of tolfenpyrad and deltamethrin on the intestinal metabolome of Glyphodes pyloalis

Author of the article:LIANG Jing1, 2** RONG Wan-Tao2 XUE Sheng-Hao2 LIANG En-Xi2 ZENG Hui-Ling2 WU Zheng-Jun1*** LI Xiao-Dong1, 2***

Author's Workplace：1. Key Laboratory of Ecology and Environmental Protection of Rare and Endangered Animals and Plants, Ministry of Education, College of Life Sciences, Guangxi Normal University, Guilin 541006, China; 2. Guangxi Key Laboratory of Sericulture Ecology and Intelligent Technology Application, College of Chemistry and Bioengineering, Hechi University, Hechi 546300, China

Key Words：Glyphodes pyloalis; tolfenpyrad; deltamethrin; pesticide; metabolomics; metabolic pathways

Abstract：[Aim] To investigate the effects of two commonly used insecticides, tolfenpyrad and deltamethrin, on the intestinal metabolites of the Glyphodes pyloalis. [Methods] A total of three G. pyloalis treatment groups were established; a non-pesticide treatment control group (DD), a tolfenpyrad treatment group (DC) and a deltamethrin treatment group (DX). Intestinal metabolites were analyzed by ultra-high performance liquid chromatography-tandem, electrostatic, field orbitrap, mass spectrometry (UHPLC-Q Exactive HFX, Thermo, USA). Data were processed using progenesis QI software. Principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and orthogonal partial least squares discriminant analysis (OPLS-DA), were used to screen differential metabolites (screening criteria: P<0.05, FC > 1.5 or < 0.667). KEGG metabolic pathway enrichment analysis was also conducted. [Results] There were significant differences in the intestinal metabolites of G. pyloalis among the different treatment groups. Compared to the DD group, 74 and 70 significantly differential metabolites were identified in the DC and DX groups, respectively, with 35 common, differential metabolites shared between these groups (5 upregulated and 30 downregulated). The differential metabolites were mainly attributed to glycerophospholipids, pyrimidine nucleosides, organic oxygenates, carboxylic acids and their derivatives. Pathway enrichment analysis showed that differential metabolites in the DC and DX groups were mainly enriched in the carbohydrate, amino acid and nucleotide, metabolisms. [Conclusion] Tolfenpyrad and deltamethrin can specifically disturb the intestinal metabolic spectrum of G. pyloalis, mainly by interfering with related metabolic pathways, such as amino acids and carbohydrates. These characteristic metabolic changes may be important physiological responses of G. pyloalis to pesticide stress.