A mini-review on the technique of insect glutathione S-transferases purification
Author of the article:TANG Fang1, 2;GAO Xi-Wu2
Author's Workplace：1. College of Forestry Resources and Environment, Nanjing Forestry University, Nanjing 210037, China; 2.Department of Entomology, China Agricultural University, Beijing 100094, China
Key Words：insect glutathione;S-transferases;isozyme; purification
Abstract：The main purification methods of insect glutathione S-transferases include fractionation, chromatography and electrophoresis. Fractionation usually is usedbefore chromatography and its purification factor is lower, while purification factor of chromatography is higher. Electrophoresis is used as an assistance purification method. Using these methods, the highest purification factor of insect glutathione S-transferase is 1 138 fold (Ma et al.purified glutathione S-transferases of Asian core borer Ostrinia furnacalis by using chromatography and electrophoresis). At last, the applications of purification of insect glutathione S-transferases are discussed.