Genetic diversity of different geographical populations of Chilo suppressalis (Walker) in China by AFLP
Author of the article:LUO GuangHua2ZHANG ZhiChun2WANG LiHua2YANG Qiong2FANG JiChao1, 2
Author's Workplace：1. College of Plant Protection, Nanjing Agricultural University, Nanjing210095, China; 2.Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing210014, China;3.Jiangsu Lixiahe Institute of Agricultural Science, Yangzhou225007, China
Key Words：Chilo suppressalis; geographic population; AFLP; genetic diversity
Abstract：DNA molecular markers by amplified fragment length polymorphism (AFLP) were applied to detect the genetic variation of 13 geographical populations of Chilo suppressalis collected from 11 provinces of China. A toltal of 445 distinct alleles were amplified by three AFLP primer pairs from C. suppressalis genomic DNA, of which 386 alleles (86.8%) were found to be polymorphic. The coefficient of gene differentiation between the populations was 0.74. There was high genetic identity between the 13 populations with the value greater than 0.67. Cluster analysis showed that all the tested populations could be grouped into 4 clades based on polymorphism of AFLP markers, of which the northern Huai river plain clade including GY, FN and FNing populations, the east-south region clade including YX and MH populations, and the Jing-Xiang-Chuang plain clade including JZ, SY and DY populations are credible in that the populations within each of the clades are consistently subject to similar geographic climate and near distance. However, the lone population with longer genetic distance to all other tested populations was probably clustered into a random clade. So the sampled populations for genetic polymorphism clustering should be as many as possible and dispersed