Cloning and sequence analysis of the Wolbachia wsp gene to detect of Wolbachia in Cydia pomonella
Author of the article:FENG Hong-Zu1, 3** WANG Yue-Ping1 CAO Yu1 YANG Ming-Lu1 LIU Hui-Ming1 XU Jian-Jun2
Author's Workplace：1. College of Plant Science, Tarim University, Alar 843300, China; 2. Institute of Plant Protection,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China; 3. Key Laboratory of Integrated Pest Management in NanJiang, Alar 843300, China
Key Words：Cydia pomonella, Wolbachia, wsp, sequence analysis, phylogeny
Abstract： [Objectives] The evolution position of Wolbachia in Cydia pomonella (Lepidoptera:Tortricidae) was identified by molecular biology in order to further provide theoretic basis for the regulatory mechanisms of reproductive role of Wolbachia. [Methods] Wolbachia infection in ten populations of Cydia pomonella were detected by using a specific primer for the Wolbachia wsp gene and PCR. These populations were from Yili, Tulufan, Hetian, Shihezi, Kuitun, Hami, Korla, Alar and Kashi in Xinjiang and Zhangye in Gansu. The Wolbachia wsp gene of from Alar populations of C. pomonella was amplified and sequenced. [Results] All populations were infected with Wolbachia. Using the specific primers, a 617 bp region of the Wolbachia wsp gene (GenBank accesstion number is KC832324) was amplified from Alar populations. Phylogenetic analysis showed that Wolbachia in C. pomonella belongs to the Dor subgroup of the A group, and had the closest genetic relationship to Trichopria drosophilae. [Conclusion] Wolbachia infection was more common in the C. pomonella, and belong to Dor subgroup in A group.