Identification and evolution of the Or83b olfactory gene of Chouioia cunea Yang
Author of the article:ZHAO Yan-Ni** LIU Qiang LI Min***
Author's Workplace：Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal University. 300387, Tianjin
Key Words：Chouioia cunea, Or83b gene, olfaction molecular mechanism, evolution analysis
Abstract：[Objectives] To investigate the olfactory molecular mechanism of Chouioia cunea Yang (Hymenoptera:Eulophidae). C. cunea is a significant parasitic natural enemy of the fall webworm Hyphantria cunea (Drury). We here provide the first description of the full-length cDNA of the Or83b orthologous gene of C. cune, named CcOr1. [Methods] We identified the full-length of CcOr1 and analyzed its gene sequence and likely evolution. [Results] Sequencing showed that CcOr1 is 1 482 bp in length, encoding 475 amino acid residues. The secondary structure of CcOr1 is similar to the G protein-coupled receptors of vertebrates with seven transmembrane regions．[Conclusion] The CcOr1 gene is highly conserved relative to other insects and appears to be primarily negatively selected. As a parasitic wasp with a wide range of hosts, C. cunea has lower synonymous substitutions and higher codon usage bias compared with host-specific wasps.