Sequence comparison and phylogenetic analysis of mtDNA CO Igene sequence fragments in Monochamus species(Coleoptera: Cerambycidae)
Author of the article:ZHENG Si-Zhu1** ZHANG Kai2*** YANG Xiao-Jun3 XU Mei3 AN Yu-Lin3
Author's Workplace：1. Laboratory for Prevention and Control of Alien Pests, Suzhou Entry-Exit Inspection and Quarantine Bureau, Suzhou 215000, China; 2. Shanghai Gongqing Forest Park, Shanghai 200000, China; 3. Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing 210001, China
Key Words： Monochamus, mtDNACOⅠgene, genetic distance, phylogenetic tree
Abstract：
To determine the molecular phylogeny of Monochamus spp. by analyzing variation in mitochondrial COⅠgene sequences. [Methods] Mitochondrial COⅠgene sequences from 11 species were analyzed using MEGA 5.0. Phylogenetic trees for Monochamus were constructed using both the Parsimony (MP) and Bayesian-Inference (BI) methods. [Result] Sequence analysis results indicate that, after alignment, 470 sites consisted of 169 variable sites (36.0%) and 301 conserved sites (64.0%). The transition versus transversion ratio (R-value) was 1 .03. Saturation plots indicate that combined analysis had better a linear relationship than the single gene analysis, suggesting that base mutations have not reached saturation. Phylogenetic trees based on MP and BI methods had similar topologies, which was consistent with the results of the traditional morphology. Our results support the taxonomic validity of the genus Monochamus. [Conclusion] This study confirms that sequence analysis is an effective method for studying the taxonomy of Monochamus.