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Issue:ISSN 2095-1353
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Your Position :Home->Past Journals Catalog->2015年52 No.2

Development and application of quarantine insectDNA barcoding technology
Author of the article:YE Jian-Ling1** AN Yu-Lin1*** QIAN Lu1 YANG Xiao-Jun1 XU Mei1WU Cui-Ping1 WEI Chun-Yan2 NIU Le
Author's Workplace:1. Jiangsu EntryExit Inspection and Quarantine Bureau, Nanjing 210001, China;. Jilin EntryExit Inspection and Quarantine Bureau, Changchun 130062, China; 3. Nanjing Helin Technology Co. Ltd., Nanjing 210037, China; 4. Nanjing Forestry University, Nanjing 210037, China
Key Words: DNA barcoding, plant quarantine, combination of nucleotide diagnostic sites, identification characteristic
Abstract:[Objectives]  DNA barcoding has been one of the main focuses of insect taxonomic research in recent years and has become a powerful tool for the identification of plant quarantine insects. In order to accurately identify and intercept pest insects at ports of entry, we developed an insect DNA barcoding identification kit (IDK). This kit allows the quick and accurate identification of known, and potential, pest insects. [Methods]  New primers for specific insect genera or groups were designed to improve amplification efficiency. A combination of nucleotide diagnostic sites from molecular sequences of closely related species from the same genera was generated and a database was built specifically for species identification. [Results]  We took quarantine insects Pissodes for the example, the combination of nucleotide diagnostic sites (identification characteristics) of 5 Pissodes species were generated, and the database was bulit for species identification. One unidentified sample of Pissodes was compared to the identification characteristics stored in the library. Similarity between the query sequence and P. terminalis in database is 100%. This identification was confirmed by later morphological examination. This paper introduces the principles, technical methods, identification process and application of this new kit, and discusses its prospective benefits for plant quarantine. [Conclusion] Insect DNA barcoding identification kit will lay a basis for establishment of standard and accurate species identification platform. It will has a good prospect of application.
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