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Issue:ISSN 2095-1353
           CN 11-6020/Q
Director:Chinese Academy of Sciences
Sponsored by:Chinese Society of Entomological;institute of zoology, chinese academy of sciences;
Address:Chaoyang District No. 1 Beichen West Road, No. 5 hospital,Beijing City,100101, China
Your Position :Home->Past Journals Catalog->2015年52 No.2

RNA-Seq (quantitative) analysis of the stress gene inDrosophila cells treated with parathion
Author of the article:LI Na** CHEN Zi LIU Wei CHI Qing-Ping HU Jun-Li CHENG Luo-Gen***
Author's Workplace:School of Life Sciences, Nanjing Normal University, Nanjing 210023, China
Key Words:parathion, cytotoxicity, RNA-Seq, qRT-PCR, Drosophila’s cells

[Objectives]  To investigate stress gene expression, and quantitative changes in the expression of this gene, in Drosophila S2 cells treated with parathion, and provide a basis for further analysis of insect stress reactions and molecular toxicology. [Methods]  The cytotoxicity and cell survival rate of Drosophila S2 cells were tested using CC-8 reagents and a cell count plate. Application of RNA-Seq and qRT-PCR to detect differentially expressed genes and quantitative changes in gene expression. [Results]  Cells exposed to 35 μg/mL parathion had high survival rates. Analysis of changes in gene expression with RNA-Seq revealed that 481 genes were up-regulated and that 52 displayed significant changes. These included oxidative stress genes, detoxification genes and immune related genes. [Conclusion]  Identifying genes that are differentially expressed in response to cytotoxicity lays the foundation for analyzing the acting targets of parathion and the mechanism underlying resistance to this pesticide.

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