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Your Position :Home->Past Journals Catalog->2015年52 No.2

Establishment of a microinjection based RNA interference technique for Bemisia tabaci (Hemiptera: Aleyrodidae)
Author of the article:LI Ru-Mei** WANG Shao-Li ZHANG You-Jun***
Author's Workplace:Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Key Words:RNA interference, microinjection, Bemisia tabaci, double strand rna, quantitative Real-time PCR
Abstract:  RNAi is a powerful tool for revealing gene function. Microinjection is the most widely-used method to obtain systematic interference by injecting dsRNA into insects. However, it’s very difficult to inject small insects, especially the sweetpotato whitefly, Bemisia tabaci. In order to verify the large-scale gene function of B. tabaci, it’s necessary to develop an RNAi technique. We established a method to silence the B. tabaci CYP6CM1 gene, the effect of which was detected by injecting the 4th instar larvae of whiteflies with different dsRNA concentrations. The results show that injection with different concentrations of dsRNA had various silencing effects on the CYP6CM1 gene, with a maximum silencing efficiency of more than 80%. This successful RNAi technology successfully can be used on other whiteflies.
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