Effects of the Bax channel on cantharidin induced apoptosis of Spodoptera frugiperda Sf9 cells
Author of the article:CUI Gao-Feng** ZHANG Peng-Fei HAO Shao-Dong DING Kai WANG Jin-Zhong ZHANG Zhi-Yong***
Author's Workplace:College of Plant Science and Technology, Beijing University of Agriculture
Key Words:cantharidin, Spodoptera frugiperda, Bax channel, cell morphology, succinate dehydrogenase, mitochondrial membrane potential
Abstract:[Objectives] To investigate the role of
the Bax channel on cantharidin-induced cell apoptosis in lepidopteran insects [Methods] Bax channel blocker was used to determine the
roles of mitochondrial membrane potential, succinate dehydrogenase activity in
mitochondria, and cell morphology, on apoptosis of Sf9 cells derived from fall
the armyworm, Spodoptera frugiperda (J.E.
Smith) exposed to cantharidin stress. [Results] The results show that Bax channel blocker can delay the decrease of
mitochondrial membrane potential and reduce changes in cell morphology,
but fail to stop the decline of succinate dehydrogenase activity, in Sf9
cells after treatment with cantharidin. [Conclusion] Bax
related channels play a role in cantharidin-induced changes in
mitochondrial membrane potential and destruction of cell morphology. However,
the Bax channel does not appear to play a role in the suppression of enzyme
activity in mitochondria after cantharidin treatment.